Construct: shRNA TRCN0000447331

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009282.3-2524s21c1
DNA Barcode:
AGAGGATTTGTTGGTATTGAG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Stag1 (20842)
Gene Description:
stromal antigen 1
Transcript:
RefSeq NM_009282.3 (CURRENT)
Match location:
Position 2524 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000447331)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGAGGATTTGTTGGTATTGAG
Hairpin Sequence:
5'-CCGG-AGAGGATTTGTTGGTATTGAG-CTCGAG-CTCAATACCAACAAATCCTCT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGAGGATTTGTTGGTATTGAGCTCGAGCTCAATACCAACAAATCCTCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGAGGATTTGTTGGTATTGAGCTCGAGCTCAATACCAACAAATCCTCT-3'

Other clones with same target sequence:

(none)