Construct: shRNA TRCN0000447371

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_026536.1-522s21c1
DNA Barcode:
CGTCATTGCTCTGCGACATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Atp5s (68055)
Gene Description:
ATP synthase, H+ transporting, mitochondrial F0 complex, subunit S
Transcript:
RefSeq NM_026536.1 (CURRENT)
Match location:
Position 522 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000447371)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGTCATTGCTCTGCGACATTT
Hairpin Sequence:
5'-CCGG-CGTCATTGCTCTGCGACATTT-CTCGAG-AAATGTCGCAGAGCAATGACG-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGTCATTGCTCTGCGACATTTCTCGAGAAATGTCGCAGAGCAATGACGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGTCATTGCTCTGCGACATTTCTCGAGAAATGTCGCAGAGCAATGACG-3'

Other clones with same target sequence:

(none)