Construct: shRNA TRCN0000448316
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_172378.2-2185s21c1
- DNA Barcode:
- GATCAGCAGTTACGGATATAT
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- Slc22a22 (210463)
- Gene Description:
- solute carrier family 22 (organic cation transporter), member 22
- Transcript:
- RefSeq NM_172378.2 (CURRENT)
- Match location:
- Position 2185 (3UTR)
Vector Information:
- Vector Backbone:
- pLKO_005
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000448316)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- GATCAGCAGTTACGGATATAT
- Hairpin Sequence:
-
5'-CCGG-GATCAGCAGTTACGGATATAT-CTCGAG-ATATATCCGTAACTGCTGATC-TTTTTTGAAT-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGGATCAGCAGTTACGGATATATCTCGAGATATATCCGTAACTGCTGATCTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAGATCAGCAGTTACGGATATATCTCGAGATATATCCGTAACTGCTGATC-3'
Other clones with same target sequence:
(none)
