Construct: shRNA TRCN0000448523

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_024329.5-889s21c1
DNA Barcode:
TCTGACGGGACCACTACTAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
EFHD2 (79180)
Gene Description:
EF-hand domain family member D2
Transcript:
RefSeq NM_024329.5 (NON-CURRENT)
Match location:
Position 889 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000448523)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCTGACGGGACCACTACTAAA
Hairpin Sequence:
5'-CCGG-TCTGACGGGACCACTACTAAA-CTCGAG-TTTAGTAGTGGTCCCGTCAGA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCTGACGGGACCACTACTAAACTCGAGTTTAGTAGTGGTCCCGTCAGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCTGACGGGACCACTACTAAACTCGAGTTTAGTAGTGGTCCCGTCAGA-3'

Other clones with same target sequence:

(none)