Construct: shRNA TRCN0000448675

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_138782.2-2667s21c1
DNA Barcode:
AGCTGACTACAAACATTAAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
FCHO2 (115548)
Gene Description:
FCH and mu domain containing endocytic adaptor 2
Transcript:
RefSeq NM_138782.2 (NON-CURRENT)
Match location:
Position 2667 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000448675)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGCTGACTACAAACATTAAAT
Hairpin Sequence:
5'-CCGG-AGCTGACTACAAACATTAAAT-CTCGAG-ATTTAATGTTTGTAGTCAGCT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGCTGACTACAAACATTAAATCTCGAGATTTAATGTTTGTAGTCAGCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGCTGACTACAAACATTAAATCTCGAGATTTAATGTTTGTAGTCAGCT-3'

Other clones with same target sequence:

(none)