Construct: shRNA TRCN0000448904

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001001850.1-574s21c1
DNA Barcode:
ACAGAAGTTTGAATGATTTAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
STX19 (415117)
Gene Description:
syntaxin 19
Transcript:
RefSeq NM_001001850.1 (NON-CURRENT)
Match location:
Position 574 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000448904)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACAGAAGTTTGAATGATTTAG
Hairpin Sequence:
5'-CCGG-ACAGAAGTTTGAATGATTTAG-CTCGAG-CTAAATCATTCAAACTTCTGT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACAGAAGTTTGAATGATTTAGCTCGAGCTAAATCATTCAAACTTCTGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACAGAAGTTTGAATGATTTAGCTCGAGCTAAATCATTCAAACTTCTGT-3'

Other clones with same target sequence:

(none)