Construct: shRNA TRCN0000449756

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011217.2-1707s21c1
DNA Barcode:
ACCGATTCCTTGAGTACTTAC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ptprr (19279)
Gene Description:
protein tyrosine phosphatase, receptor type, R
Transcript:
RefSeq NM_011217.2 (CURRENT)
Match location:
Position 1707 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000449756)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACCGATTCCTTGAGTACTTAC
Hairpin Sequence:
5'-CCGG-ACCGATTCCTTGAGTACTTAC-CTCGAG-GTAAGTACTCAAGGAATCGGT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACCGATTCCTTGAGTACTTACCTCGAGGTAAGTACTCAAGGAATCGGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACCGATTCCTTGAGTACTTACCTCGAGGTAAGTACTCAAGGAATCGGT-3'

Other clones with same target sequence:

(none)