Construct: shRNA TRCN0000450003

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001166066.1-1441s21c1
DNA Barcode:
ACTCTGTTAAGATCAACTATC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Mroh2b (223825)
Gene Description:
maestro heat-like repeat family member 2B
Transcript:
RefSeq NM_001166066.1 (CURRENT)
Match location:
Position 1441 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000450003)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACTCTGTTAAGATCAACTATC
Hairpin Sequence:
5'-CCGG-ACTCTGTTAAGATCAACTATC-CTCGAG-GATAGTTGATCTTAACAGAGT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACTCTGTTAAGATCAACTATCCTCGAGGATAGTTGATCTTAACAGAGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACTCTGTTAAGATCAACTATCCTCGAGGATAGTTGATCTTAACAGAGT-3'

Other clones with same target sequence:

(none)