Construct: shRNA TRCN0000450458

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009643.1-5357s21c1
DNA Barcode:
GCCTGATGTTGGGCTTAATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ahnak (66395)
Gene Description:
AHNAK nucleoprotein (desmoyokin)
Transcript:
RefSeq NM_009643.1 (NON-CURRENT)
Match location:
Position 5357 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000450458)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTGATGTTGGGCTTAATTT
Hairpin Sequence:
5'-CCGG-GCCTGATGTTGGGCTTAATTT-CTCGAG-AAATTAAGCCCAACATCAGGC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTGATGTTGGGCTTAATTTCTCGAGAAATTAAGCCCAACATCAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTGATGTTGGGCTTAATTTCTCGAGAAATTAAGCCCAACATCAGGC-3'

Other clones with same target sequence:

(none)