Construct: shRNA TRCN0000450919

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_198093.2-1117s21c1
DNA Barcode:
CACCTTCTACTTCAATCTATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Elmo1 (140580)
Gene Description:
engulfment and cell motility 1
Transcript:
RefSeq NM_198093.2 (NON-CURRENT)
Match location:
Position 1117 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000450919)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CACCTTCTACTTCAATCTATT
Hairpin Sequence:
5'-CCGG-CACCTTCTACTTCAATCTATT-CTCGAG-AATAGATTGAAGTAGAAGGTG-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCACCTTCTACTTCAATCTATTCTCGAGAATAGATTGAAGTAGAAGGTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACACCTTCTACTTCAATCTATTCTCGAGAATAGATTGAAGTAGAAGGTG-3'

Other clones with same target sequence:

(none)