Construct: shRNA TRCN0000451680

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_020490.1-720s21c1
DNA Barcode:
CCACGCGGTCAATCTCCTACA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ltb4r2 (57260)
Gene Description:
leukotriene B4 receptor 2
Transcript:
RefSeq NM_020490.1 (NON-CURRENT)
Match location:
Position 720 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000451680)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCACGCGGTCAATCTCCTACA
Hairpin Sequence:
5'-CCGG-CCACGCGGTCAATCTCCTACA-CTCGAG-TGTAGGAGATTGACCGCGTGG-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCACGCGGTCAATCTCCTACACTCGAGTGTAGGAGATTGACCGCGTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCACGCGGTCAATCTCCTACACTCGAGTGTAGGAGATTGACCGCGTGG-3'

Other clones with same target sequence:

(none)