Construct: shRNA TRCN0000452588

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001145051.1-369s21c1
DNA Barcode:
GCGGCTCAAGTACCTGCATGA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TMEM247 (388946)
Gene Description:
transmembrane protein 247
Transcript:
RefSeq NM_001145051.1 (NON-CURRENT)
Match location:
Position 369 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000452588)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCGGCTCAAGTACCTGCATGA
Hairpin Sequence:
5'-CCGG-GCGGCTCAAGTACCTGCATGA-CTCGAG-TCATGCAGGTACTTGAGCCGC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCGGCTCAAGTACCTGCATGACTCGAGTCATGCAGGTACTTGAGCCGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCGGCTCAAGTACCTGCATGACTCGAGTCATGCAGGTACTTGAGCCGC-3'

Other clones with same target sequence:

(none)