Construct: shRNA TRCN0000452943

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011632.2-842s21c1
DNA Barcode:
ACTTGTCCGAGTGTGTCAATG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Traf3 (22031)
Gene Description:
TNF receptor-associated factor 3
Transcript:
RefSeq NM_011632.2 (NON-CURRENT)
Match location:
Position 842 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000452943)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACTTGTCCGAGTGTGTCAATG
Hairpin Sequence:
5'-CCGG-ACTTGTCCGAGTGTGTCAATG-CTCGAG-CATTGACACACTCGGACAAGT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACTTGTCCGAGTGTGTCAATGCTCGAGCATTGACACACTCGGACAAGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACTTGTCCGAGTGTGTCAATGCTCGAGCATTGACACACTCGGACAAGT-3'

Other clones with same target sequence:

(none)