Construct: shRNA TRCN0000453317
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_011372.2-848s21c1
- DNA Barcode:
- GAAAGATGAGTGTAACGAATA
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- St6galnac3 (20447)
- Gene Description:
- ST6 (alpha-N-acetyl-neuraminyl-2,3-beta-galactosyl-1,3)-N-acetylgalactosaminide alpha-2,6-sialyltransferase 3
- Transcript:
- RefSeq NM_011372.2 (CURRENT)
- Match location:
- Position 848 (CDS)
Vector Information:
- Vector Backbone:
- pLKO_005
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000453317)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- GAAAGATGAGTGTAACGAATA
- Hairpin Sequence:
-
5'-CCGG-GAAAGATGAGTGTAACGAATA-CTCGAG-TATTCGTTACACTCATCTTTC-TTTTTTGAAT-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGGAAAGATGAGTGTAACGAATACTCGAGTATTCGTTACACTCATCTTTCTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAGAAAGATGAGTGTAACGAATACTCGAGTATTCGTTACACTCATCTTTC-3'
Other clones with same target sequence:
(none)
