Construct: shRNA TRCN0000454138

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_175032.2-1069s21c1
DNA Barcode:
CCCTTTGAGTCTCCGGTTATG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Galntl6 (270049)
Gene Description:
UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase-like 6
Transcript:
RefSeq NM_175032.2 (NON-CURRENT)
Match location:
Position 1069 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000454138)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCTTTGAGTCTCCGGTTATG
Hairpin Sequence:
5'-CCGG-CCCTTTGAGTCTCCGGTTATG-CTCGAG-CATAACCGGAGACTCAAAGGG-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCTTTGAGTCTCCGGTTATGCTCGAGCATAACCGGAGACTCAAAGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCTTTGAGTCTCCGGTTATGCTCGAGCATAACCGGAGACTCAAAGGG-3'

Other clones with same target sequence:

(none)