Construct: shRNA TRCN0000454141

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009325.3-751s21c1
DNA Barcode:
GGGTAGCTATGGTGTTCTTCG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tbxa2r (21390)
Gene Description:
thromboxane A2 receptor
Transcript:
RefSeq NM_009325.3 (NON-CURRENT)
Match location:
Position 751 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000454141)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GGGTAGCTATGGTGTTCTTCG
Hairpin Sequence:
5'-CCGG-GGGTAGCTATGGTGTTCTTCG-CTCGAG-CGAAGAACACCATAGCTACCC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGGGTAGCTATGGTGTTCTTCGCTCGAGCGAAGAACACCATAGCTACCCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGGGTAGCTATGGTGTTCTTCGCTCGAGCGAAGAACACCATAGCTACCC-3'

Other clones with same target sequence:

(none)