Construct: shRNA TRCN0000454564

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009644.2-1249s21c1
DNA Barcode:
GAGATGCACAAGTACAGTTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ahrr (11624)
Gene Description:
aryl-hydrocarbon receptor repressor
Transcript:
RefSeq NM_009644.2 (NON-CURRENT)
Match location:
Position 1249 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000454564)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGATGCACAAGTACAGTTAT
Hairpin Sequence:
5'-CCGG-GAGATGCACAAGTACAGTTAT-CTCGAG-ATAACTGTACTTGTGCATCTC-TTTTTTTTAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGATGCACAAGTACAGTTATCTCGAGATAACTGTACTTGTGCATCTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGATGCACAAGTACAGTTATCTCGAGATAACTGTACTTGTGCATCTC-3'

Other clones with same target sequence:

(none)