Construct: shRNA TRCN0000454912

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007034.3-316s21c1
DNA Barcode:
GAAGTATTGAGTGATCCTAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DNAJB4 (11080)
Gene Description:
DnaJ heat shock protein family (Hsp40) member B4
Transcript:
RefSeq NM_007034.3 (NON-CURRENT)
Match location:
Position 316 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000454912)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAAGTATTGAGTGATCCTAAA
Hairpin Sequence:
5'-CCGG-GAAGTATTGAGTGATCCTAAA-CTCGAG-TTTAGGATCACTCAATACTTC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAAGTATTGAGTGATCCTAAACTCGAGTTTAGGATCACTCAATACTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAAGTATTGAGTGATCCTAAACTCGAGTTTAGGATCACTCAATACTTC-3'

Other clones with same target sequence:

(none)