Construct: shRNA TRCN0000454968

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_202004.3-1309s21c1
DNA Barcode:
ATTAGGGAAAGAAGTCTATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
HJV (148738)
Gene Description:
hemojuvelin BMP co-receptor
Transcript:
RefSeq NM_202004.3 (NON-CURRENT)
Match location:
Position 1309 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000454968)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATTAGGGAAAGAAGTCTATTT
Hairpin Sequence:
5'-CCGG-ATTAGGGAAAGAAGTCTATTT-CTCGAG-AAATAGACTTCTTTCCCTAAT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATTAGGGAAAGAAGTCTATTTCTCGAGAAATAGACTTCTTTCCCTAATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATTAGGGAAAGAAGTCTATTTCTCGAGAAATAGACTTCTTTCCCTAAT-3'

Other clones with same target sequence:

(none)