Construct: shRNA TRCN0000454980

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_020958.2-365s21c1
DNA Barcode:
GGAACAAGTGTGATTGCAAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PPP4R4 (57718)
Gene Description:
protein phosphatase 4 regulatory subunit 4
Transcript:
RefSeq NM_020958.2 (CURRENT)
Match location:
Position 365 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000454980)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GGAACAAGTGTGATTGCAAAT
Hairpin Sequence:
5'-CCGG-GGAACAAGTGTGATTGCAAAT-CTCGAG-ATTTGCAATCACACTTGTTCC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGGAACAAGTGTGATTGCAAATCTCGAGATTTGCAATCACACTTGTTCCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGGAACAAGTGTGATTGCAAATCTCGAGATTTGCAATCACACTTGTTCC-3'

Other clones with same target sequence:

(none)