Construct: shRNA TRCN0000455004
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_198861.1-1260s21c1
- DNA Barcode:
- GGATGAGATCAGAGTACTAAC
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- Lrrc75a (192976)
- Gene Description:
- leucine rich repeat containing 75A
- Transcript:
- RefSeq NM_198861.1 (CURRENT)
- Match location:
- Position 1260 (3UTR)
Vector Information:
- Vector Backbone:
- pLKO_005
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000455004)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- GGATGAGATCAGAGTACTAAC
- Hairpin Sequence:
-
5'-CCGG-GGATGAGATCAGAGTACTAAC-CTCGAG-GTTAGTACTCTGATCTCATCC-TTTTTTGAAT-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGGGATGAGATCAGAGTACTAACCTCGAGGTTAGTACTCTGATCTCATCCTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAGGATGAGATCAGAGTACTAACCTCGAGGTTAGTACTCTGATCTCATCC-3'
Other clones with same target sequence:
(none)
