Construct: ORF TRCN0000471833
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016796.1_s317c1
- Derived from:
- ccsbBroadEn_03240
- DNA Barcode:
- CCTCAAGGGACCATCTCTCCGTAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- HSPA14 (51182)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471833
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51182 | HSPA14 | heat shock protein family A... | NM_016299.4 | 100% | 100% | |
| 2 | mouse | 50497 | Hspa14 | heat shock protein 14 | NM_015765.2 | 85.9% | 90.9% | (many diffs) |
| 3 | mouse | 50497 | Hspa14 | heat shock protein 14 | XM_011238980.2 | 77.9% | 78.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1596
- ORF length:
- 1527
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcggccatc ggagttcacc tgggctgcac ctcagcctgt gtggccgtct 121 ataaggatgg ccgggctggt gtggttgcaa atgatgccgg tgaccgagtt actccagctg 181 ttgttgctta ctcagaaaat gaagagattg ttggattggc agcaaaacaa agtagaataa 241 gaaatatttc aaatacagta atgaaagtaa agcagatcct gggcagaagc tccagtgatc 301 cacaagctca gaaatacatc gcggaaagta aatgtttagt cattgaaaaa aatgggaaat 361 tacgatatga aatagatact ggagaagaaa caaaatttgt taacccagaa gatgttgcca 421 gactgatatt tagtaaaatg aaagaaacgg cacattctgt attgggctca gatgcaaatg 481 atgtagttat tactgtcccg tttgattttg gagaaaagca aaaaaatgct cttggagaag 541 cagctagagc tgctggattt aatgttttgc gattaattca cgaaccgtct gcagctcttc 601 ttgcttatgg aattggacaa gactccccta ctggaaaaag caatattttg gtgtttaagc 661 ttggaggaac atccttatct ctcagcgtca tggaagttaa cagtggaata tatcgggttc 721 tttcaacaaa cactgatgat aacatcggtg gtgcacattt cacagaaacc ttagcacagt 781 atctagcttc tgagttccaa agatccttca aacatgatgt gagaggaaat gcgcgagcca 841 tgatgaaatt aacgaacagt gctgaagtag cgaaacattc tttgtcaacc ttgggaagtg 901 ccaactgttt tcttgactca ttatatgaag gtcaagattt tgattgcaat gtgtccagag 961 caagatttga acttctttgt tctccacttt ttaataagtg tatagaagca atcagaggac 1021 tcttagatca aaatggattt acagcagatg atatcaacaa ggttgtcctt tgtggagggt 1081 cttctcgaat cccaaagcta cagcaactga ttaaagatct tttcccagct gttgagcttc 1141 tcaattctaT CCCTCCTGAT GAAGTGATCC CTATTGGTGC AGCTATAGAA GCAGGAATTC 1201 TTATTGGGAA AGAAAACCTG TTGGTGGAAG ACTCTCTTAT GATAGAGTGT TCAGCCAGAG 1261 ATATTTTAGT TAAGGGTGTG GACGAATCAG GAGCCAGTAG ATTCACAGTG CTGTTTCCAT 1321 CAGGGACTCC TTTGCCAGCT CGAAGACAAC ACACATTGCA AGCCCCTGGA AGCATATCTT 1381 CAGTGTGCCT TGAACTCTAT GAGTCTGATG GGAAGAACTC TGCCAAAGAG GAAACCAAGT 1441 TTGCACAGGT TGTACTCCAG GATTTAGATA AAAAAGAAAA TGGATTACGT GATATATTAG 1501 CTGTTCTTAC TATGAAAAGG GATGGATCTT TACATGTGAC ATGCACAGAT CAAGAAACTG 1561 GAAAATGTGA AGCAATCTCT ATTGAGATAG CATCTTTGCC AACTTTCTTG TACAAAGTGG 1621 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 1681 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 1741 ACCTCAAGGG ACCATCTCTC CGTAAACGCG TTAAGTCgac aatcaacctc tggattacaa 1801 aatttgtgaa agatt