Construct: ORF TRCN0000492044
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013707.1_s317c1
- Derived from:
- ccsbBroadEn_11349
- DNA Barcode:
- ATTCCAACAAAGTTCTAGCTCCCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- VAPA (9218)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000492044
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9218 | VAPA | VAMP associated protein A | NM_194434.3 | 95.1% | 95.1% | 1_21del;733_747del |
| 2 | human | 9218 | VAPA | VAMP associated protein A | XM_017026079.1 | 91% | 89.3% | (many diffs) |
| 3 | human | 9218 | VAPA | VAMP associated protein A | NM_003574.6 | 80.6% | 80.6% | 1_21del;417_551del;868_882del |
| 4 | human | 9218 | VAPA | VAMP associated protein A | XM_011525769.3 | 77% | 75.4% | (many diffs) |
| 5 | human | 9218 | VAPA | VAMP associated protein A | XM_024451287.1 | 57.8% | 57.8% | 0_1ins213;183_317del;634_648del |
| 6 | human | 112268155 | LOC112268155 | vesicle-associated membrane... | XR_002957735.1 | 11.2% | (many diffs) | |
| 7 | mouse | 30960 | Vapa | vesicle-associated membrane... | NM_013933.3 | 89% | 92.3% | (many diffs) |
| 8 | mouse | 30960 | Vapa | vesicle-associated membrane... | XM_006524385.3 | 77.4% | 80.1% | (many diffs) |
| 9 | mouse | 30960 | Vapa | vesicle-associated membrane... | XM_017317500.1 | 76.6% | 79.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 777
- ORF length:
- 711
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc gaagcacgag cagatcctgg tcctcgatcc gcccacagac ctcaaattca 121 aaggcccctt cacagatgta gtcactacaa atcttaaatt gcgaaatcca tcggatagaa 181 aagtgtgttt caaagtgaag actacagcac ctcgccggta ctgtgtgagg cccaacagtg 241 gaattattga cccagggtca actgtgactg tttcagtaat gctacagccc tttgactatg 301 atccgaatga aaagagtaaa cacaagttta tggtacagac aatttttgct ccaccaaaca 361 cttcagatat ggaagctgtg tggAAAGAGG CAAAACCTGA TGAATTAATG GATTCCAAAT 421 TGAGATGCGT ATTTGAAATG CCCAATGAAA ATGATAAATT GAATGATATG GAACCTAGCA 481 AAGCTGTTCC ACTGAATGCA TCTAAGCAAG ATGGACCTAT GCCAAAACCA CACAGTGTTT 541 CACTTAATGA TACCGAAACA AGGAAACTAA TGGAAGAGTG TAAAAGACTT CAGGGAGAAA 601 TGATGAAGCT ATCAGAAGAA AATCGGCACC TGAGAGATGA AGGTTTAAGG CTCAGAAAGG 661 TAGCACATTC GGATAAACCT GGATCAACCT CAACTGCATC CTTCAGAGAT AATGTCACCA 721 GTCCTCTTCC TTCACTTCTT GTTGTAATTG CAGCCATTTT CATTGGATTC TTTCTAGGGA 781 AATTCATCTT GTACCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 841 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 901 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGAATT CCAACAAAGT TCTAGCTCCC 961 CACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t