Construct: shRNA TRCN0000583929

Construct Description:

Construct Type:
shRNA
Other Identifiers:
n/a
DNA Barcode:
TCTCCCTCATTGACCTAAATT

Original Target:

Taxon:
n/a
Gene:
n/a
Gene Description:
n/a
Transcript:
n/a
Match location:
n/a

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000583929)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCTCCCTCATTGACCTAAATT
Hairpin Sequence:
5'-CCGG-TCTCCCTCATTGACCTAAATT-CTCGAG-AATTTAGGTCAATGAGGGAGA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCTCCCTCATTGACCTAAATTCTCGAGAATTTAGGTCAATGAGGGAGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCTCCCTCATTGACCTAAATTCTCGAGAATTTAGGTCAATGAGGGAGA-3'

Other clones with same target sequence:

(none)