Construct: shRNA TRCN0000583931

Construct Description:

Construct Type:
shRNA
Other Identifiers:
n/a
DNA Barcode:
CCACTAATGAATCCTATAACA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
OR51B5 (282763)
Gene Description:
olfactory receptor family 51 subfamily B member 5
Transcript:
RefSeq NM_001005567.2 (NON-CURRENT)
Match location:
Position 1294 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000583931)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCACTAATGAATCCTATAACA
Hairpin Sequence:
5'-CCGG-CCACTAATGAATCCTATAACA-CTCGAG-TGTTATAGGATTCATTAGTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCACTAATGAATCCTATAACACTCGAGTGTTATAGGATTCATTAGTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCACTAATGAATCCTATAACACTCGAGTGTTATAGGATTCATTAGTGG-3'

Other clones with same target sequence:

(none)