Construct: shRNA TRCN0000583936

Construct Description:

Construct Type:
shRNA
Other Identifiers:
n/a
DNA Barcode:
TCCACTACCCAACCATAATGA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
OR6B1 (135946)
Gene Description:
olfactory receptor family 6 subfamily B member 1
Transcript:
RefSeq NM_001005281.1 (NON-CURRENT)
Match location:
Position 389 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000583936)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCCACTACCCAACCATAATGA
Hairpin Sequence:
5'-CCGG-TCCACTACCCAACCATAATGA-CTCGAG-TCATTATGGTTGGGTAGTGGA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCCACTACCCAACCATAATGACTCGAGTCATTATGGTTGGGTAGTGGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCCACTACCCAACCATAATGACTCGAGTCATTATGGTTGGGTAGTGGA-3'

Other clones with same target sequence:

(none)