Construct: shRNA TRCN0000583993

Construct Description:

Construct Type:
shRNA
Other Identifiers:
n/a
DNA Barcode:
TCATTCTCTTTGTTCTCATTT

Original Target:

Taxon:
n/a
Gene:
n/a
Gene Description:
n/a
Transcript:
n/a
Match location:
n/a

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000583993)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCATTCTCTTTGTTCTCATTT
Hairpin Sequence:
5'-CCGG-TCATTCTCTTTGTTCTCATTT-CTCGAG-AAATGAGAACAAAGAGAATGA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCATTCTCTTTGTTCTCATTTCTCGAGAAATGAGAACAAAGAGAATGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCATTCTCTTTGTTCTCATTTCTCGAGAAATGAGAACAAAGAGAATGA-3'

Other clones with same target sequence:

(none)