Construct: shRNA TRCN0000584708

Construct Description:

Construct Type:
shRNA
Other Identifiers:
n/a
DNA Barcode:
CTGCGTGGCTCAGCTCTATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
OR10G7 (390265)
Gene Description:
olfactory receptor family 10 subfamily G member 7
Transcript:
RefSeq NM_001004463.1 (CURRENT)
Match location:
Position 285 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000584708)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTGCGTGGCTCAGCTCTATTT
Hairpin Sequence:
5'-CCGG-CTGCGTGGCTCAGCTCTATTT-CTCGAG-AAATAGAGCTGAGCCACGCAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTGCGTGGCTCAGCTCTATTTCTCGAGAAATAGAGCTGAGCCACGCAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTGCGTGGCTCAGCTCTATTTCTCGAGAAATAGAGCTGAGCCACGCAG-3'

Other clones with same target sequence:

(none)