Construct: shRNA TRCN0000584806

Construct Description:

Construct Type:
shRNA
Other Identifiers:
n/a
DNA Barcode:
ATTTGGCTGTCTCCCTATCTC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
OR7E24 (26648)
Gene Description:
olfactory receptor family 7 subfamily E member 24
Transcript:
RefSeq NM_001079935.1 (CURRENT)
Match location:
Position 669 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000584806)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATTTGGCTGTCTCCCTATCTC
Hairpin Sequence:
5'-CCGG-ATTTGGCTGTCTCCCTATCTC-CTCGAG-GAGATAGGGAGACAGCCAAAT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATTTGGCTGTCTCCCTATCTCCTCGAGGAGATAGGGAGACAGCCAAATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATTTGGCTGTCTCCCTATCTCCTCGAGGAGATAGGGAGACAGCCAAAT-3'

Other clones with same target sequence:

(none)