Construct: ORF ccsbBroad304_00082
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003048.1_s304c1, BRDN0000460264
- Derived from:
- ccsbBroadEn_00082
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- APOC4 (346)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_00082
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 346 | APOC4 | apolipoprotein C4 | NM_001646.3 | 100% | 100% | |
| 2 | human | 100533990 | APOC4-APOC2 | APOC4-APOC2 readthrough (NM... | NR_037932.1 | 20.8% | 1_40del;422_1829del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 450
- ORF length:
- 381
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gtccctcctc agaaacaggc tccaggccct gcctgccctg tgcctctgcg 121 tgctggtcct ggcctgcatt ggggcatgcc agccagaggc ccaggaagga accctgagcc 181 ccccaccaaa gctaaagatg agtcgctgga gcctggtgag gggcaggatg aaggagctgc 241 tggagacagt ggtgaacagg accagagacg ggtggcaatg gttctggagc ccgagcacct 301 tccggggctt catgcagacc tactatgacg accacctgag ggacctgggt ccgctcacca 361 aggcctggtt cctcgaatcc aaagacagcc tcttgaagaa gacccacagc ctgtgcccca 421 ggcttgtctg tggggacaag gaccagggtt tgccaacttt cttgtacaaa gtggttggta 481 agcctatccc taaccctctc ctcggtctcg attctacgta gtaatgagct agcgctaacc 541 ggtggcgcgt taagtcgaca atcaacctct ggatta