Construct: ORF ccsbBroad304_00155
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015238.1_s304c1, BRDN0000408131
- Derived from:
- ccsbBroadEn_00155
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CCND1 (595)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_00155
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 595 | CCND1 | cyclin D1 | NM_053056.3 | 100% | 100% | |
| 2 | mouse | 12443 | Ccnd1 | cyclin D1 | NM_007631.2 | 90.1% | 93.8% | (many diffs) |
| 3 | mouse | 12443 | Ccnd1 | cyclin D1 | XM_011241977.1 | 83.9% | 87.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 218
- ORF end:
- 1103
- ORF length:
- 885
- Sequence:
-
1 TTCATTCTCC TTGTTGTGGG TTGTTTTTTC CTTTGTTTTT TCCCCCTTCC ATTTTTGATT 61 TAAGCAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAACT CGAGGCATCT 121 ATGTCGGGTG CGGAGAAAGA GGTAATGAAA TGGCACATGG TCATAGCTGT TTCCTGACta 181 actgtcggga tcaacaagtt tgtacaaaaa agttgccatg gaacaccagc tcctgtgctg 241 cgaagtggaa accatccgcc gcgcgtaccc cgatgccaac ctcctcaacg accgggtgct 301 gcgggccatg ctgaaggcgg aggagacctg cgcgccctcg gtgtcctact tcaaatgtgt 361 gcagaaggag gtcctgccgt ccatgcggaa gatcgtcgcc acctggatgc tggaggtctg 421 cgaggaacag aagtgcgagg aggaggtctt cccgctggcc atgaactacc tggaccgctt 481 cctgtcgctg gagcccgtga aaaagagccg cctgcagctg ctgggggcca cttgcatgtt 541 cgtggcctct aagatgaagg agaccatccc cctgacggcc gagaagctgt gcatctacac 601 cgacaactcc atccggcccg aggagctgct gcaaatggag ctgctcctgg tgaacaagct 661 caagtggaac ctggccgcaa tgaccccgca cgatttcatt gaacacttcc tctccaaaat 721 gccagaggcg gaggagaaca aacagatcat ccgcaaacac gcgcagacct tcgttgccct 781 ctgtgccaca gatgtgaagt tcatttccaa tccgccctcc atggtggcag cggggagcgt 841 ggtggccgca gtgcaaggcc tgaacctgag gagccccaac aacttcctgt cctactaccg 901 cctcacacgc ttcctctcca gagtgatcaa gtgtgacccg gactgcctcc gggcctgcca 961 ggagcagatc gaagccctgc tggagtcaag cctgcgccag gcccagcaga acatggaccc 1021 caaggccgcc gaggaggagg aagaggagga ggaggaggtg gacctggctt gcacacccac 1081 cgacgtgcgg gacgtggaca tctgcccaac tttcttgtac aaagtggttg gtaagcctat 1141 ccctaaccct ctcctcggtc tcgattctac gtagtaatga gctagcgcta accggtggcg 1201 cgttaagtcg acaatcaacc tctggatta