Construct: ORF ccsbBroad304_00276
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013293.1_s304c1, BRDN0000409943
- Derived from:
- ccsbBroadEn_00276
- DNA Barcode:
- None
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- CDK2 (1017)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_00276
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1017 | CDK2 | cyclin dependent kinase 2 | NM_001798.5 | 99.8% | 30.1% | 236delT |
| 2 | human | 1017 | CDK2 | cyclin dependent kinase 2 | NM_052827.4 | 88.4% | 33.9% | 236delT;485_486ins102 |
| 3 | human | 1017 | CDK2 | cyclin dependent kinase 2 | XM_011537732.2 | 86% | 25.9% | 236delT;589_732del |
| 4 | human | 1017 | CDK2 | cyclin dependent kinase 2 | NM_001290230.2 | 79.7% | 24.1% | 116_117ins78;158delT;407_408ins102 |
| 5 | mouse | 12566 | Cdk2 | cyclin-dependent kinase 2 | NM_016756.4 | 92.3% | 29.7% | (many diffs) |
| 6 | mouse | 12566 | Cdk2 | cyclin-dependent kinase 2 | NM_183417.3 | 79.5% | 25.6% | (many diffs) |
| 7 | mouse | 12566 | Cdk2 | cyclin-dependent kinase 2 | XM_006513162.2 | 55.8% | 3.4% | (many diffs) |
| 8 | mouse | 12566 | Cdk2 | cyclin-dependent kinase 2 | XM_011243321.2 | 55.8% | 3.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 408
- ORF length:
- 342
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgga gaacttccaa aaggtggaaa agatcggaga gggcacgtac ggagttgtgt 121 acaaagccag aaacaagttg acgggagagg tggtggcgct taagaaaatc cgcctggaca 181 ctgagactga gggtgtgccc agtactgcca tccgagagat ctctctgctt aaggagctta 241 accatcctaa tattgtcaag ctgctggatg tcattcacac agAAAATAAA CTCTACCTGG 301 TTTTGAATTT CTGCACCAAG ATCTCAAGAA ATTCATGGAT GCCTCTGCTC TCACTGGCAT 361 TCCTCTTCCC CTCATCAAGA GCTATCTGTT CCAGCTGCTC CAGGGCCTAG CTTTCTGCCA 421 TTCTCATCGG GTCCTCCACC GAGACCTTAA ACCTCAGAAT CTGCTTATTA ACACAGAGGG 481 GGCCATCAAG CTAGCAGACT TTGGACTAGC CAGAGCTTTT GGAGTCCCTG TTCGTACTTA 541 CACCCATGAG GTGGTGACCC TGTGGTACCG AGCTCCTGAA ATCCTCCTGG GCTGCAAATA 601 TTATTCCACA GCTGTGGACA TCTGGAGCCT GGGCTGCATC TTTGCTGAGA TGGTGACTCG 661 CCGGGCCCTA TTCCCTGGAG ATTCTGAGAT TGACCAGCTC TTCCGGATCT TTCGGACTCT 721 GGGGACCCCA GATGAGGTGG TGTGGCCAGG AGTTACTTCT ATGCCTGATT ACAAGCCAAG 781 TTTCCCCAAG TGGGCCCGGC AAGATTTTAG TAAAGTTGTA CCTCCCCTGG ATGAAGATGG 841 ACGGAGCTTG TTATCGCAAA TGCTGCACTA CGACCCTAAC AAGCGGATTT CGGCCAAGGC 901 AGCCCTGGCT CACCCTTTCT TCCAGGATGT GACCAAGCCA GTACCCCATC TTCGACTCTG 961 CCCAACTTTC TTGTACaaag tggttggtaa gcctatccct aaccctctcc tcggtctcga 1021 ttctacgtag taatgagcta gcgctaaccg gtggcgcgtt aagtcgacaa tcaacctctg 1081 gatta