Construct: ORF ccsbBroad304_00278
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010134.1_s304c1, BRDN0000408969
- Derived from:
- ccsbBroadEn_00278
- DNA Barcode:
- None
- Epitope Tag:
- V5 (not translated due to frame shift)
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CDK5 (1020)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_00278
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1020 | CDK5 | cyclin dependent kinase 5 | NM_004935.4 | 100% | 100% | |
| 2 | human | 1020 | CDK5 | cyclin dependent kinase 5 | NM_001164410.3 | 89% | 89% | 311_312ins96 |
| 3 | mouse | 12568 | Cdk5 | cyclin-dependent kinase 5 | NM_007668.3 | 90.8% | 99.6% | (many diffs) |
| 4 | mouse | 12568 | Cdk5 | cyclin-dependent kinase 5 | XM_006535626.2 | 83.4% | 90.4% | (many diffs) |
| 5 | mouse | 12568 | Cdk5 | cyclin-dependent kinase 5 | XR_389739.3 | 33% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 942
- ORF length:
- 876
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 tnggcatgca gaaatacgag aaactggaaa agattgggga aggcacctac ggaactgtgt 121 tcaaggccaa aaaccgggag actcatgaga tcgtggctct gaaacgggtg aggctggatg 181 acgatgatga gggtgtgccg agttccgccc tccgggagat ctgcctactc aaggagctga 241 agcacaagaa catcgtcagg cttcatgacg tcctgcacag cgacaagaag ctgactttgg 301 tttttgaatt ctgtgaccag gacctgaaga agtattttga cagttgcaat ggtgacctcg 361 atcctgagat tgtaaagtca ttcctcttcc agctactaaa agggctggga ttctgtcata 421 gccgcaatgt gctacacagg gacctgaagc cccagaacct gctaataaac aggaatgggg 481 agctgaaatt ggctgatttt ggcctggctc gagcctttgg gattcccgtc cgctgttact 541 cagctgaggt ggtcacactg tggtaccgcc caccggatgt cctctttggg gccaagctgt 601 actccacgtc catcgacatg tggtcagccg gctgcatctt tgcagagctg gccaatgctg 661 ggcggcctct ttttcccggc aatgatgtcg atgaccagtt gaagaggatc ttccgactgc 721 tggggacgcc caccgaggag cagtggccct ctatgaccaa gctgccagac tataagccct 781 atccgatgta cccggccaca acatccctgg tgaacgtcgt gcccaaactc aatgccacag 841 ggagggatct gctgcagaac cttctgaagt gtaaccctgt ccagcgtatc tcagcagaag 901 aggccctgca gcacccctac ttctccgact tctgtccgcc ctcccaactt tcttgtacaa 961 agtggttggt aagcctatcc ctaaccctct cctcggtctc gattctacgt agtaatgagc 1021 tagcgctaac cggtggcgcg ttaagtcgac aatcaacctc tggatta