Construct: ORF ccsbBroad304_00291
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010733.1_s304c1, BRDN0000405750
- Derived from:
- ccsbBroadEn_00291
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CENPA (1058)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_00291
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1058 | CENPA | centromere protein A | NM_001042426.1 | 100% | 100% | |
| 2 | human | 1058 | CENPA | centromere protein A | NM_001809.4 | 81.4% | 81.4% | 210_287del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 408
- ORF length:
- 342
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggg cccgcgccgc cggagccgaa agcccgaggc cccgaggagg cgcagcccga 121 gcccgacccc gacccccggc ccctcccggc ggggcccctc cttaggcgct tcctcccatc 181 aacacagtcg gcggagacaa ggttggctaa aggagatccg aaagcttcag aagagcacac 241 acctcttgat aaggaagctg cccttcagcc gcctggcagc agaagcattt ctagttcatc 301 tctttgagga cgcctatctc ctcaccttac atgcaggccg agttactctc ttcccaaagg 361 atgtgcaact ggcccggagg atccggggcc ttgaggaggg actcggctgc ccaactttct 421 tgtacaaagt ggttggtaag cctatcccta accctctcct cggtctcgat tctacgtagt 481 aatgagctag cgctaaccgg tggcgcgtta agtcgacaat caacctctgg atta