Construct: ORF ccsbBroad304_00424
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009097.1_s304c1, BRDN0000410814
- Derived from:
- ccsbBroadEn_00424
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DBI (1622)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_00424
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1622 | DBI | diazepam binding inhibitor,... | NM_001178042.3 | 100% | 100% | |
| 2 | human | 1622 | DBI | diazepam binding inhibitor,... | NM_001282633.2 | 100% | 100% | |
| 3 | human | 1622 | DBI | diazepam binding inhibitor,... | NM_001282634.2 | 100% | 100% | |
| 4 | human | 1622 | DBI | diazepam binding inhibitor,... | NM_001282635.2 | 100% | 100% | |
| 5 | human | 1622 | DBI | diazepam binding inhibitor,... | NM_020548.8 | 100% | 100% | |
| 6 | human | 1622 | DBI | diazepam binding inhibitor,... | NM_001178043.3 | 90.1% | 80.7% | (many diffs) |
| 7 | human | 1622 | DBI | diazepam binding inhibitor,... | NM_001079863.2 | 83.3% | 80.7% | (many diffs) |
| 8 | human | 1622 | DBI | diazepam binding inhibitor,... | NM_001079862.3 | 83% | 80.7% | 1_1delAins29;4T>C;7_8ins23 |
| 9 | human | 1622 | DBI | diazepam binding inhibitor,... | NM_001178041.3 | 75.6% | 65.1% | (many diffs) |
| 10 | human | 1622 | DBI | diazepam binding inhibitor,... | NM_001178017.3 | 66% | 57.1% | (many diffs) |
| 11 | human | 1622 | DBI | diazepam binding inhibitor,... | NM_001282636.2 | 60.5% | 60.5% | 0_1ins123 |
| 12 | human | 1622 | DBI | diazepam binding inhibitor,... | NM_001352432.2 | 60.5% | 60.5% | 0_1ins123 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 378
- ORF length:
- 312
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgtg gggcgacctc tggctcctcc cgcctgcctc tgccaatccg ggcactggga 121 cagaggctga gtttgagaaa gctgcagagg aggttaggca ccttaagacc aagccatcgg 181 atgaggagat gctgttcatc tatggccact acaaacaagc aactgtgggc gacataaata 241 cagaacggcc cgggatgttg gacttcacgg gcaaggccaa gtgggatgcc tggaatgagc 301 tgaaagggac ttccaaggaa gatgccatga aagcttacat caacaaagta gaagagctaa 361 agaaaaaata cgggatatgc ccaactttct tgtacaaagt ggttggtaag cctatcccta 421 accctctcct cggtctcgat tctacgtagt aatgagctag cgctaaccgg tggcgcgtta 481 agtcgacaat caacctctgg atta