Construct: ORF ccsbBroad304_00478
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015243.1_s304c1, BRDN0000407654
- Derived from:
- ccsbBroadEn_00478
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- S1PR3 (1903)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_00478
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1903 | S1PR3 | sphingosine-1-phosphate rec... | NM_005226.4 | 100% | 100% | |
| 2 | mouse | 13610 | S1pr3 | sphingosine-1-phosphate rec... | NM_010101.4 | 85.1% | 87.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1200
- ORF length:
- 1134
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggc aactgccctc ccgccgcgtc tccagccggt gcgggggaac gagaccctgc 121 gggagcatta ccagtacgtg gggaagttgg cgggcaggct gaaggaggcc tccgagggca 181 gcacgctcac caccgtgctc ttcttggtca tctgcagctt catcgtcttg gagaacctga 241 tggttttgat tgccatctgg aaaaacaata aatttcacaa ccgcatgtac tttttcattg 301 gcaacctggc tctctgcgac ctgctggccg gcatcgctta caaggtcaac attctgatgt 361 ctggcaagaa gacgttcagc ctgtctccca cggtctggtt cctcagggag ggcagtatgt 421 tcgtggccct tggggcgtcc acctgcagct tactggccat cgccatcgag cggcacttga 481 caatgatcaa aatgaggcct tacgacgcca acaagaggca ccgcgtcttc ctcctgatcg 541 ggatgtgctg gctcattgcc ttcacgctgg gcgccctgcc cattctgggc tggaactgcc 601 tgcacaatct ccctgactgc tctaccatcc tgcccctcta ctccaagaag tacattgcct 661 tctgcatcag catcttcacg gccatcctgg tgaccatcgt gatcctctac gcacgcatct 721 acttcctggt gaagtccagc agccgtaagg tggccaacca caacaactcg gagcggtcca 781 tggcactgct gcggaccgtg gtgattgtgg tgagcgtgtt catcgcctgc tggtccccac 841 tcttcatcct cttcctcatt gatgtggcct gcagggtgca ggcgtgcccc atcctcttca 901 aggctcagtg gttcatcgtg ttggctgtgc tcaactccgc catgaacccg gtcatctaca 961 cgctggccag caaggagatg cggcgggcct tcttccgtct ggtctgcaac tgcctggtca 1021 ggggacgggg ggcccgcgcc tcacccatcc agcctgcgct cgacccaagc agaagtaaat 1081 caagcagcag caacaatagc agccactctc cgaaggtcaa ggaagacctg ccccacacag 1141 ccccctcatc ctgcatcatg gacaagaacg cagcacttca gaatgggatc ttctgcaact 1201 gcccaacttt cttgtacaaa gtggttggta agcctatccc taaccctctc ctcggtctcg 1261 attctacgta gtaatgagct agcgctaacc ggtggcgcgt taagtcgaca atcaacctct 1321 ggatta