Construct: ORF ccsbBroad304_00630
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018122.1_s304c1, BRDN0000399006
- Derived from:
- ccsbBroadEn_00630
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GFAP (2670)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_00630
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2670 | GFAP | glial fibrillary acidic pro... | NM_002055.5 | 100% | 100% | |
| 2 | human | 2670 | GFAP | glial fibrillary acidic pro... | NM_001242376.2 | 92.4% | 88.7% | (many diffs) |
| 3 | human | 2670 | GFAP | glial fibrillary acidic pro... | NM_001131019.3 | 92% | 89.9% | (many diffs) |
| 4 | human | 2670 | GFAP | glial fibrillary acidic pro... | NM_001363846.1 | 91.5% | 91.5% | 1171_1290del |
| 5 | human | 2670 | GFAP | glial fibrillary acidic pro... | XM_024450692.1 | 86.4% | 86.4% | 520_723del |
| 6 | human | 2670 | GFAP | glial fibrillary acidic pro... | XM_024450691.1 | 80.2% | 76.9% | (many diffs) |
| 7 | human | 2670 | GFAP | glial fibrillary acidic pro... | XM_024450690.1 | 80% | 80% | 520_723del;1375_1494del |
| 8 | human | 2670 | GFAP | glial fibrillary acidic pro... | XM_024450693.1 | 79.8% | 77.9% | (many diffs) |
| 9 | mouse | 14580 | Gfap | glial fibrillary acidic pro... | NM_010277.3 | 86.7% | 90.7% | (many diffs) |
| 10 | mouse | 14580 | Gfap | glial fibrillary acidic pro... | NM_001131020.1 | 80.8% | 81.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1362
- ORF length:
- 1296
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 gtggcatgga gaggagacgc atcacctccg ctgctcgccg ctcctacgtc tcctcagggg 121 agatgatggt ggggggcctg gctcctggcc gccgtctggg tcctggcacc cgcctctccc 181 tggctcgaat gccccctcca ctcccgaccc gggtggattt ctccctggct ggggcactca 241 atgctggctt caaggagacc cgggccagtg agcgggcaga gatgatggag ctcaatgacc 301 gctttgccag ctacatcgag aaggttcgct tcctggaaca gcaaaacaag gcgctggctg 361 ctgagctgaa ccagctgcgg gccaaggagc ccaccaagct ggcagacgtc taccaggctg 421 agctgcgaga gctgcggctg cggctcgatc aactcaccgc caacagcgcc cggctggagg 481 ttgagaggga caatctggca caggacctgg ccactgtgag gcagaagctc caggatgaaa 541 ccaacctgag gctggaagcc gagaacaacc tggctgccta tagacaggaa gcagatgaag 601 ccaccctggc ccgtctggat ctggagagga agattgagtc gctggaggag gagatccggt 661 tcttgaggaa gatccacgag gaggaggttc gggaactcca ggagcagctg gcccgacagc 721 aggtccatgt ggagcttgac gtggccaagc cagacctcac cgcagccctg aaagagatcc 781 gcacgcagta tgaggcaatg gcgtccagca acatgcatga agccgaagag tggtaccgct 841 ccaagtttgc agacctgaca gacgctgctg cccgcaacgc ggagctgctc cgccaggcca 901 agcacgaagc caacgactac cggcgccagt tgcagtcctt gacctgcgac ctggagtctc 961 tgcgcggcac gaacgagtcc ctggagaggc agatgcgcga gcaggaggag cggcacgtgc 1021 gggaggcggc cagttatcag gaggcgctgg cgcggctgga ggaagagggg cagagcctca 1081 aggacgagat ggcccgccac ttgcaggagt accaggacct gctcaatgtc aagctggccc 1141 tggacatcga gatcgccacc tacaggaagc tgctagaggg cgaggagaac cggatcacca 1201 ttcccgtgca gaccttctcc aacctgcaga ttcgagaaac cagcctggac accaagtctg 1261 tgtcagaagg ccacctcaag aggaacatcg tggtgaagac cgtggagatg cgggatggag 1321 aggtcattaa ggagtccaag caggagcaca aggatgtgat gtgcccaact ttcttgtaca 1381 aagtggttgg taagcctatc cctaaccctc tcctcggtct cgattctacg tagtaatgag 1441 ctagcgctaa ccggtggcgc gttaagtcga caatcaacct ctggatta