Construct: ORF ccsbBroad304_00725

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF014844.1_s304c1, BRDN0000410442
Derived from:
ccsbBroadEn_00725
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
HADHA (3030)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_00725
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 3030 HADHA hydroxyacyl-CoA dehydrogena... NM_000182.5 100% 100%
2 mouse 97212 Hadha hydroxyacyl-Coenzyme A dehy... NM_178878.2 85.8% 86.5% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2355
ORF length:
2289
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatggt ggcctgccgg gcgattggca tcctcagccg cttttctgcc ttcaggatcc
121tccgctcccg aggttatata tgccgcaatt ttacagggtc ttctgctttg ctgaccagaa
181cccatattaa ctatggagtc aaaggggatg tggcagttgt tcgaattaac tctcccaatt
241caaaggtaaa tacactgagt aaagagctac attcagagtt ctcagaagtt atgaatgaaa
301tctgggctag tgatcaaatc agaagtgccg tccttatctc atcaaagcca ggctgcttta
361ttgcaggtgc tgatatcaac atgttagccg cttgcaagac ccttcaagaa gtaacacagc
421tatcacaaga agcacagaga atagttgaga aacttgaaaa gtccacaaag cctattgtgg
481ctgccatcaa tggatcctgc ctgggaggag gacttgaggt tgccatttca tgccaataca
541gaatagcaac aaaagacaga aaaacagtat taggtacccc tgaagttttg ctgggggcct
601taccaggagc aggaggcaca caaaggctgc ccaaaatggt gggtgtgcct gctgctttgg
661acatgatgct gactggtaga agcattcgtg cagacagggc aaagaaaatg ggactggttg
721accaactggt ggaacccctg ggaccaggac taaaacctcc agaggaacgg acaatagaat
781acctagaaga agttgcaatt acttttgcca aaggactagc tgataagaag atctctccaa
841agagagacaa gggattggtg gaaaaattga cagcgtatgc catgactatt ccatttgtca
901ggcaacaggt ttacaaaaaa gtggaagaaa aagtgcgaaa gcagactaaa ggcctttatc
961ctgcacctct gaaaataatt gatgtggtaa agactggaat tgagcaaggg agtgatgccg
1021gttatctctg tgaatctcag aaatttggag agcttgtaat gaccaaagaa tcaaaggcct
1081tgatgggact ctaccatggt caggtcctgt gcaagaagaa taaatttgga gctccacaga
1141aggatgttaa gcatctggct attcttggtg cagggctgat gggagcaggc atcgcccaag
1201tctccgtgga taaggggcta aagactatac ttaaagatgc caccctcact gcgctagacc
1261gaggacagca acaagtgttc aaaggattga atgacaaagt gaagaagaaa gctctaacat
1321catttgaaag ggattccatc ttcagcaact tgactgggca gcttgattac caaggttttg
1381aaaaggccga catggtgatt gaagctgtgt ttgaggacct tagtcttaag cacagagtgc
1441taaaggaagt agaagcggtg attccagatc actgtatctt tgccagtaac acatctgctc
1501tcccaatcag tgaaatcgct gctgtcagca aaagacctga gaaggtgatt ggcatgcact
1561acttctctcc cgtggacaag atgcagctgc tggagattat cacgaccgag aaaacttcca
1621aagacaccag tgcttcagct gtagcagttg gtctcaagca ggggaaggtc atcattgtgg
1681ttaaggatgg acctggcttc tatactacca ggtgtcttgc gcccatgatg tctgaagtca
1741tccgaatcct ccaggaagga gttgacccga agaagctgga ttccctgacc acaagctttg
1801gctttcctgt gggtgccgcc acactggtgg atgaagttgg tgtggatgta gcgaaacatg
1861tggcggaaga tctgggcaaa gtctttgggg agcggtttgg aggtggaaac ccagaactgc
1921tgacacagat ggtgtccaag ggcttcctag gtcgtaaatc tgggaagggc ttttacatct
1981atcaggaggg tgtgaagagg aaggatttga attctgacat ggatagtatt ttagcgagtc
2041tgaagctgcc tcctaagtct gaagtctcat cagacgaaga catccagttc cgcctggtga
2101caagatttgt gaatgaggca gtcatgtgcc tgcaagaggg gatcttggcc acacctgcag
2161agggagacat cggagccgtc tttgggcttg gcttcccgcc ttgtctggga gggcctttcc
2221gctttgtgga tctgtatggc gcccagaaga tagtggaccg gctcaagaaa tatgaagctg
2281cctatggaaa acagttcacc ccatgccagc tgctagctga ccatgctaac agccctaaca
2341agaagttcta ccagtgccca actttcttgt acaaagtggt tggtaagcct atccctaacc
2401ctctcctcgg tctcgattct acgtagtaat gagctagcgc taaccggtgg cgcgttaagt
2461cgacaatcaa cctctggatt a

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