Construct: ORF ccsbBroad304_00734
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011722.1_s304c1, BRDN0000403253
- Derived from:
- ccsbBroadEn_00734
- DNA Barcode:
- None
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- HDAC1 (3065)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_00734
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3065 | HDAC1 | histone deacetylase 1 | NM_004964.3 | 99.9% | 60.6% | 894delG |
| 2 | human | 3065 | HDAC1 | histone deacetylase 1 | XM_011541309.2 | 59.8% | 22.5% | 0_1ins579;315delG |
| 3 | mouse | 433759 | Hdac1 | histone deacetylase 1 | NM_008228.2 | 90.3% | 60.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1113
- ORF length:
- 1047
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggc gcagacgcag ggcacccgga ggaaagtctg ttactactac gacggggatg 121 ttggaaatta ctattatgga caaggccacc caatgaagcc tcaccgaatc cgcatgactc 181 ataatttgct gctcaactat ggtctctacc gaaaaatgga aatctatcgc cctcacaaag 241 ccaatgctga ggagatgacc aagtaccaca gcgatgacta cattaaattc ttgcgctcca 301 tccgtccaga taacatgtcg gagtacagca agcagatgca gagattcaac gttggtgagg 361 actgtccagt attcgatggc ctgtttgagt tctgtcagtt gtctactggt ggttctgtgg 421 caagtgctgt gaaacttaat aagcagcaga cggacatcgc tgtgaattgg gctgggggcc 481 tgcaccatgc aaagaagtcc gaggcatctg gcttctgtta cgtcaatgat atcgtcttgg 541 ccatcctgga actgctaaag tatcaccaga gggtgctgta cattgacatt gatattcacc 601 atggtgacgg cgtggaagag gccttctaca ccacggaccg ggtcatgact gtgtcctttc 661 ataagtatgg agagtacttc ccaggaactg gggacctacg ggatatcggg gctggcaaag 721 gcaagtatta tgctgttaac tacccgctcc gagacgggat tgatgacgag tcctatgagg 781 ccattttcaa gccggtcatg tccaaagtaa tggagatgtt ccagcctagt gcggtggtct 841 tacagtgtgg ctcagactcc ctatctgggg atcggttagg ttgcttcaat ctaactatca 901 aaggacacgc caagtgtgtg gaaTTTGTCA AGAGCTTTAA CCTGCCTATG CTGATGCTGG 961 AGGCGGTGGT TACACCATTC GTAACGTTGC CCGGTGCTGG ACATATGAGA CAGCTGTGGC 1021 CCTGGATACG GAGATCCCTA ATGAGCTTCC ATACAATGAC TACTTTGAAT ACTTTGGACC 1081 AGATTTCAAG CTCCACATCA GTCCTTCCAA TATGACTAAC CAGAACACGA ATGAGTACCT 1141 GGAGAAGATC AAACAGCGAC TGTTTGAGAA CCTTAGAATG CTGCCGCACG CACCTGGGGT 1201 CCAAATGCAG GCGATTCCTG AGGACGCCAT CCCTGAGGAG AGTGGCGATG AGGACGAAGA 1261 CGACCCTGAC AAGCGCATCT CGATCTGCTC CTCTGACAAA CGAATTGCCT GTGAGGAAGA 1321 GTTCTCCGAT TCTGAAGAGG AGGGAGAGGG GGGCCGCAAG AACTCTTCCA ACTTCAAAAA 1381 AGCCAAGAGA GTCAAAACAG AGGATGAAAA AGAGAAAGAC CCAGAGGAGA AGAAAGAAGT 1441 CACCGAAGAG GAGAAAACCA AGGAGGAGAA GCCAGAAGCC AAAGGGGTCA AGGAGGAGGT 1501 CAAGTTGGCC TGCCCAACTT TCTTGTACaa agtggttggt aagcctatcc ctaaccctct 1561 cctcggtctc gattctacgt agtaatgagc tagcgctaac cggtggcgcg ttaagtcgac 1621 aatcaacctc tggatta