Construct: ORF ccsbBroad304_00786
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000773.1_s304c1, BRDN0000461667
- Derived from:
- ccsbBroadEn_00786
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- HRC (3270)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_00786
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3270 | HRC | histidine rich calcium bind... | NM_002152.3 | 100% | 100% | |
| 2 | human | 3270 | HRC | histidine rich calcium bind... | XM_017026733.2 | 79.2% | 78.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2163
- ORF length:
- 2097
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggg ccaccatagg ccatggctgc acgcttctgt cctctgggct ggggtggcca 121 gcctgctcct ccccccggcc atgacccagc agctcagagg ggatgggcta ggcttcagaa 181 accggaacaa cagcactgga gtcgccgggc tctccgagga ggcatcagca gagcttcgcc 241 accacctcca cagccctaga gaccatccag atgagaacaa ggatgtttcc acagagaatg 301 ggcatcattt ctggagccac ccagaccgtg aaaaggagga tgaagatgtc tccaaggaat 361 atgggcacct actcccaggc cacaggtccc aagaccacaa agtcggagat gagggtgtct 421 caggtgagga ggtctttgca gagcatggtg ggcaggcccg tgggcacaga ggccacggga 481 gtgaagacac ggaagactca gctgagcaca ggcaccacct ccccagccac aggagccaca 541 gccatcaaga cgaggatgag gatgaagttg tgtccagtga gcatcaccat catatcctca 601 ggcatggaca ccgaggccat gatggggaag atgatgaagg agaagaggag gaggaggagg 661 aggaggagga agaggaggcc tccactgagt atggacacca ggcccacagg caccgaggcc 721 atgggagtga agaggatgag gatgtctcag atggacacca tcatcatggc cccagccaca 781 ggcaccaagg ccatgaagaa gatgacgatg atgatgatga tgatgatgat gatgatgatg 841 atgatgatgt ctccattgaa tatagacacc aggctcacag gcaccaaggc cacgggattg 901 aagaggatga agatgtctca gatggacacc atcatcgcga ccccagccac aggcaccgaa 961 gccatgaaga agatgacaat gatgatgatg atgtctccac tgagtatgga caccaggccc 1021 acaggcacca agaccacaga aaggaagagg ttgaggctgt ctcaggtgaa caccaccatc 1081 atgtccctga ccacaggcac caaggccaca gagacgagga agaagatgag gatgtgtcca 1141 ctgaacgttg gcaccagggt ccccaacatg tccaccatgg ccttgtagat gaggaagagg 1201 aagaagagga gatcacagtc cagttcggcc actatgttgc aagccaccaa cctcgaggcc 1261 acaagagtga tgaagaggac ttccaagatg agtataaaac agaagtccct caccatcacc 1321 accacagagt ccccagggag gaagatgagg aggtctctgc tgagcttggc caccaggccc 1381 ccagccacag gcaaagccac caagatgaag aaactggcca tggtcaaaga gggtccatca 1441 aagagatgag ccatcacccc ccaggacaca cagtggtcaa ggatagaagc catttgagga 1501 aggatgattc tgaggaggaa aaggagaagg aagaggaccc cggctcccat gaggaagacg 1561 atgaaagttc agagcaggga gagaaaggca cccatcatgg cagccgggac caggaagatg 1621 aggaggatga ggaggaaggt catggcctca gcctgaacca ggaggaggaa gaagaggaag 1681 acaaggagga ggaggaggag gaagaagacg aggagaggag ggaagagagg gctgaggttg 1741 gggccccact gagcccagac cacagcgagg aggaagagga ggaggaggag gggctggagg 1801 aagatgagcc ccgcttcacc atcatcccca acccactgga caggagagag gaggctggag 1861 gtgcctccag cgaggaggaa agcggtgagg acacaggtcc acaggatgct caggagtatg 1921 ggaactacca gccagggtcc ctgtgtggct actgctcctt ctgcaatcga tgcactgaat 1981 gtgagagctg tcactgtgat gaggagaaca tgggtgagca ctgcgaccag tgccagcact 2041 gtcagttctg ctatctctgc ccgctggtct gcgaaacggt ctgcgctcca ggaagctacg 2101 ttgactattt ctcctcgtcc ctttatcagg ccctggcaga catgctggaa acgccggaac 2161 cctgcccaac tttcttgtac aaagtggttg gtaagcctat ccctaaccct ctcctcggtc 2221 tcgattctac gtagtaatga gctagcgcta accggtggcg cgttaagtcg acaatcaacc 2281 tctggatta