Construct: ORF ccsbBroad304_01066
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009112.1_s304c1, BRDN0000461757
- Derived from:
- ccsbBroadEn_01066
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NDUFA4 (4697)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_01066
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4697 | NDUFA4 | NDUFA4 mitochondrial comple... | NM_002489.4 | 100% | 100% | |
| 2 | mouse | 17992 | Ndufa4 | NADH dehydrogenase (ubiquin... | NM_010886.2 | 85.3% | 87.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 312
- ORF length:
- 243
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gctccgccag atcatcggtc aggccaagaa gcatccgagc ttgatccccc 121 tctttgtatt tattggaact ggagctactg gagcaacact gtatctcttg cgtctggcat 181 tgttcaatcc agatgtttgt tgggacagaa ataacccaga gccctggaac aaactgggtc 241 ccaatgatca atacaagttc tactcagtga atgtggatta cagcaagctg aagaaggaac 301 gtccagattt cttgccaact ttcttgtaca aagtggttgg taagcctatc cctaaccctc 361 tcctcggtct cgattctacg tagtaatgag ctagcgctaa ccggtggcgc gttaagtcga 421 caatcaacct ctggatta