Construct: ORF ccsbBroad304_01072
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008432.1_s304c1, BRDN0000402689
- Derived from:
- ccsbBroadEn_01072
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NDUFB6 (4712)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_01072
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4712 | NDUFB6 | NADH:ubiquinone oxidoreduct... | NM_002493.5 | 100% | 100% | |
| 2 | human | 4712 | NDUFB6 | NADH:ubiquinone oxidoreduct... | NM_182739.3 | 88.2% | 88.2% | 271_272ins45 |
| 3 | human | 4712 | NDUFB6 | NADH:ubiquinone oxidoreduct... | NM_001199987.1 | 75.7% | 75.7% | 180_181ins93 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 450
- ORF length:
- 384
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgac ggggtacact ccggatgaga aactgcggct gcagcagctg cgagagctga 121 gaaggcgatg gctgaaggac caggagctga gccctcggga gccggtgctg cccccacaga 181 agatggggcc tatggagaaa ttctggaata aatttttgga gaataaatcc ccttggagga 241 aaatggtcca tggggtatac aaaaagagta tctttgtttt cactcatgta cttgtacctg 301 tctggattat tcattattac atgaagtatc atgtttctga aaaaccatat ggcatagttg 361 aaaagaagtc cagaatattc cctggtgata caattctgga gactggagaa gtaattccac 421 caatgaaaga atttcctgat caacatcatt acccaacttt cttgtacaaa gtggttggta 481 agcctatccc taaccctctc ctcggtctcg attctacgta gtaatgagct agcgctaacc 541 ggtggcgcgt taagtcgaca atcaacctct ggatta