Construct: ORF ccsbBroad304_01149
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006061.1_s304c1, BRDN0000400627
- Derived from:
- ccsbBroadEn_01149
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PCBP1 (5093)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_01149
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5093 | PCBP1 | poly(rC) binding protein 1 | NM_006196.4 | 100% | 100% | |
| 2 | human | 5094 | PCBP2 | poly(rC) binding protein 2 | NM_001128913.1 | 77.6% | 80.7% | (many diffs) |
| 3 | human | 5094 | PCBP2 | poly(rC) binding protein 2 | NM_001098620.3 | 77% | 79.6% | (many diffs) |
| 4 | mouse | 23983 | Pcbp1 | poly(rC) binding protein 1 | NM_011865.4 | 94.1% | 100% | (many diffs) |
| 5 | mouse | 18521 | Pcbp2 | poly(rC) binding protein 2 | XM_006520584.3 | 77.3% | 80.7% | (many diffs) |
| 6 | mouse | 18521 | Pcbp2 | poly(rC) binding protein 2 | NM_001103166.1 | 76.7% | 79.6% | (many diffs) |
| 7 | mouse | 18521 | Pcbp2 | poly(rC) binding protein 2 | XM_017316500.1 | 76.5% | 79.3% | (many diffs) |
| 8 | mouse | 18521 | Pcbp2 | poly(rC) binding protein 2 | NM_001174073.1 | 73.9% | 77.3% | (many diffs) |
| 9 | mouse | 18521 | Pcbp2 | poly(rC) binding protein 2 | XM_006520585.3 | 73.9% | 77.3% | (many diffs) |
| 10 | mouse | 18521 | Pcbp2 | poly(rC) binding protein 2 | XM_017316501.1 | 73.8% | 77.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1134
- ORF length:
- 1068
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgga tgccggtgtg actgaaagtg gactaaatgt gactctcacc attcggcttc 121 ttatgcacgg aaaggaagta ggaagcatca ttgggaagaa aggggagtcg gttaagagga 181 tccgcgagga gagtggcgcg cggatcaaca tctcggaggg gaattgtccg gagagaatca 241 tcactctgac cggccccacc aatgccatct ttaaggcttt cgctatgatc atcgacaagc 301 tggaggaaga tatcaacagc tccatgacca acagtaccgc ggccagcagg cccccggtca 361 ccctgaggct ggtggtgccg gccacccagt gcggctccct gattgggaaa ggcgggtgta 421 agatcaaaga gatccgcgag agtacggggg cgcaggtcca ggtggcgggg gatatgctgc 481 ccaactccac cgagcgggcc atcaccatcg ctggcgtgcc gcagtctgtc accgagtgtg 541 tcaagcagat ttgcctggtc atgctggaga cgctctccca gtctccgcaa gggagagtca 601 tgaccattcc gtaccagccc atgccggcca gctccccagt catctgcgcg ggcggccaag 661 atcggtgcag cgacgctgcg ggctaccccc atgccaccca tgacctggag ggaccacctc 721 tagatgccta ctcgattcaa ggacaacaca ccatttctcc gctcgatctg gccaagctga 781 accaggtggc aagacaacag tctcactttg ccatgatgca cggcgggacc ggattcgccg 841 gaattgactc cagctctcca gaggtgaaag gctattgggc aagtttggat gcatctactc 901 aaaccaccca tgaactcacc attccaaata acttaattgg ctgcataatc gggcgccaag 961 gcgccaacat taatgagatc cgccagatgt ccggggccca gatcaaaatt gccaacccag 1021 tggaaggctc ctctggtagg caggttacta tcactggctc tgctgccagt attagtctgg 1081 cccagtatct aatcaatgcc aggctttcct ctgagaaggg catggggtgc agctacccaa 1141 ctttcttgta caaagtggtt ggtaagccta tccctaaccc tctcctcggt ctcgattcta 1201 cgtagtaatg agctagcgct aaccggtggc gcgttaagtc gacaatcaac ctctggatta