Construct: ORF ccsbBroad304_01256

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF003404.1_s304c1, BRDN0000405642
Derived from:
ccsbBroadEn_01256
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
PPM1G (5496)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_01256
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 5496 PPM1G protein phosphatase, Mg2+/M... NM_177983.3 100% 100%
2 mouse 14208 Ppm1g protein phosphatase 1G (for... NM_008014.3 91.5% 93.9% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1704
ORF length:
1638
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatggg tgcctacctc tcccagccca acacggtgaa gtgctccggg gacggggtcg
121gcgccccgcg cctgccgctg ccctacggct tctccgccat gcaaggctgg cgcgtctcca
181tggaggatgc tcacaactgt attcctgagc tggacagtga gacagccatg ttttctgtct
241acgatggaca tggaggggag gaagttgcct tgtactgtgc caaatatctt cctgatatca
301tcaaagatca gaaggcctac aaggaaggca agctacagaa ggctttagaa gatgccttct
361tggctattga cgccaaattg accactgaag aagtcattaa agagctggca cagattgcag
421ggcgacccac tgaggatgaa gatgaaaaag aaaaagtagc tgatgaagat gatgtggaca
481atgaggaggc tgcactgctg catgaagagg ctaccatgac tattgaagag ctgctgacac
541gctacgggca gaactgtcac aagggccctc cccacagcaa atctggaggt gggacaggcg
601aggaaccagg gtcccagggc ctcaatgggg aggcaggacc tgaggactca actagggaaa
661ctccttcaca agaaaatggc cccacagcca aggcctacac aggcttttcc tccaactcgg
721aacgtgggac tgaggcaggc caagttggtg agcctggcat tcccactggt gaggctgggc
781cttcctgctc ttcagcctct gacaagctgc ctcgagttgc taagtccaag ttctttgagg
841acagtgagga tgagtcagat gaggcggagg aagaagagga agacagtgag gaatgcagcg
901aggaagagga tggctacagc agtgaggagg cagagaatga ggaagatgag gatgacaccg
961aggaggctga agaggacgat gaagaagaag aagaagagat gatggtgcca gggatggaag
1021gcaaagagga gcctggctct gacagtggta caacagcggt ggtggccctg atacgaggga
1081agcagttgat tgtagccaac gcaggagact ctcgctgtgt ggtatctgag gctggcaaag
1141ctttagacat gtcctatgat cacaaaccag aggatgaagt agaactagca cgcatcaaga
1201atgctggtgg caaggtcacc atggatgggc gagtcaacgg gggcctcaac ctctccagag
1261ccattgggga ccacttctat aagagaaaca agaacctgcc acctgaggaa cagatgattt
1321cagcccttcc tgacatcaag gtgctgactc tcactgacga ccatgaattc atggtcattg
1381cctgtgatgg catctggaat gtgatgagca gccaggaagt tgtagatttc attcaatcaa
1441agatcagcca gcgtgatgaa aatggggagc ttcggttatt gtcatccatt gtggaagagc
1501tgctggatca gtgcctggca ccagacactt ctggggatgg tacagggtgt gacaacatga
1561cctgcatcat catttgcttc aagccccgaa acacagcaga gctccagcca gagagtggca
1621agcgaaaact agaggaggtg ctctctactg agggggctga agaaaatggc aacagcgaca
1681agaagaagaa ggccaagcga gactacccaa ctttcttgta caaagtggtt ggtaagccta
1741tccctaaccc tctcctcggt ctcgattcta cgtagtaatg agctagcgct aaccggtggc
1801gcgttaagtc gacaatcaac ctctggatta

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