Construct: ORF ccsbBroad304_01983
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006162.1_s304c1, BRDN0000408802
- Derived from:
- ccsbBroadEn_01983
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- EIF3D (8664)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_01983
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8664 | EIF3D | eukaryotic translation init... | NM_003753.4 | 100% | 100% | |
| 2 | human | 8664 | EIF3D | eukaryotic translation init... | NR_156418.2 | 84.6% | 1_101del;960_1021del;1808_1942del | |
| 3 | mouse | 55944 | Eif3d | eukaryotic translation init... | NM_018749.2 | 91.1% | 98.9% | (many diffs) |
| 4 | mouse | 55944 | Eif3d | eukaryotic translation init... | XM_006521192.1 | 91.1% | 98.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1713
- ORF length:
- 1644
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcaaagttc atgacacccg tgatccagga caacccctca ggctggggtc 121 cctgtgcggt tcccgagcag tttcgggata tgccctacca gccgttcagc aaaggagatc 181 ggctaggaaa ggttgcagac tggacaggag ccacatacca agataagagg tacacaaata 241 agtactcctc tcagtttggt ggtggaagtc aatatgctta tttccatgag gaggatgaaa 301 gtagcttcca gctggtggat acagcgcgca cacagaagac ggcctaccag cggaatcgaa 361 tgagatttgc ccagaggaac ctccgcagag acaaagatcg tcggaacatg ttgcagttca 421 acctgcagat cctgcctaag agtgccaaac agaaagagag agaacgcatt cgactgcaga 481 aaaagttcca gaaacaattt ggggttaggc agaaatggga tcagaaatca cagaaacccc 541 gagactcttc agttgaagtt cgtagtgatt gggaagtgaa agaggaaatg gattttcctc 601 agttgatgaa gatgcgctac ttggaagtat cagagccaca ggacattgag tgttgtgggg 661 ccctagaata ctacgacaaa gcctttgacc gcatcaccac gaggagtgag aagccactgc 721 ggagcatcaa gcgcatcttc cacactgtca ccaccacaga cgaccctgtc atccgcaagc 781 tggcaaaaac tcaggggaat gtgtttgcca ctgatgccat cctggccacg ctgatgagct 841 gtacccgctc agtgtattcc tgggatattg tcgtccagag agttgggtcc aaactcttct 901 ttgacaagag agacaactct gactttgacc tcctgacagt gagtgagact gccaatgagc 961 cccctcaaga tgaaggtaat tccttcaatt caccccgcaa cctggccatg gaggcaacct 1021 acatcaacca caatttctcc cagcagtgct tgagaatggg gaaggaaaga tacaacttcc 1081 ccaacccaaa cccgtttgtg gaggacgaca tggataagaa tgaaatcgcc tctgttgcgt 1141 accgttaccg caggtggaag cttggagatg atattgacct tattgtccgt tgtgagcacg 1201 atggcgtcat gactggagcc aacggggaag tgtccttcat caacatcaag acactcaatg 1261 agtgggattc caggcactgt aatggcgttg actggcgtca gaagctggac tctcagcgag 1321 gggctgtcat tgccacggag ctgaagaaca acagctacaa gttggcccgg tggacctgct 1381 gtgctttgct ggctggatct gagtacctca agcttggtta tgtgtctcgg taccacgtga 1441 aagactcctc acgccacgtc atcctaggca cccagcagtt caagcctaat gagtttgcca 1501 gccagatcaa cctgagcgtg gagaatgcct ggggcatttt acgctgcgtc attgacatct 1561 gcatgaagct ggaggagggc aaatacctca tcctcaagga ccccaacaag caggtcatcc 1621 gtgtctacag cctccctgat ggcaccttca gctctgatga agatgaggag gaagaggagg 1681 aggaagaaga ggaagaagaa gaggaagaaa ctttgccaac tttcttgtac aaagtggttg 1741 gtaagcctat ccctaaccct ctcctcggtc tcgattctac gtagtaatga gctagcgcta 1801 accggtggcg cgttaagtcg acaatcaacc tctggatta