Construct: ORF ccsbBroad304_02101
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001167.1_s304c1, BRDN0000409046
- Derived from:
- ccsbBroadEn_02101
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DDX21 (9188)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_02101
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9188 | DDX21 | DExD-box helicase 21 | NM_004728.4 | 100% | 100% | |
| 2 | human | 9188 | DDX21 | DExD-box helicase 21 | XM_011540336.1 | 93.1% | 93.1% | 1384_1385ins162 |
| 3 | human | 9188 | DDX21 | DExD-box helicase 21 | NM_001256910.1 | 91.3% | 91.3% | 0_1ins204 |
| 4 | human | 9188 | DDX21 | DExD-box helicase 21 | XM_017016910.2 | 84.4% | 84.4% | 0_1ins204;1180_1181ins162 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2415
- ORF length:
- 2349
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgcc gggaaaactc cgtagtgacg ctggtttgga atcagacacc gcaatgaaaa 121 aaggggagac actgcgaaag caaaccgagg agaaagagaa aaaagagaag ccaaaatctg 181 ataagactga agagatagca gaagaggaag aaactgtttt ccccaaagct aaacaagtta 241 aaaagaaagc agagccttct gaagttgaca tgaattctcc taaatccaaa aaggcaaaaa 301 agaaagagga gccatctcaa aatgacattt ctcctaaaac caaaagtttg agaaagaaaa 361 aggagcccat tgaaaagaaa gtggtttctt ctaaaaccaa aaaagtgaca aaaaatgagg 421 agccttctga ggaagaaata gatgctccta agcccaagaa gatgaagaaa gaaaaggaaa 481 tgaatggaga aactagagag aaaagcccca aactgaagaa tggatttcct catcctgaac 541 cggactgtaa ccccagtgaa gctgccagtg aagaaagtaa cagtgagata gagcaggaaa 601 tacctgtgga acaaaaagaa ggcgctttct ctaattttcc catatctgaa gaaactatta 661 aacttctcaa aggccgagga gtgaccttcc tatttcctat acaagcaaag acattccatc 721 atgtttacag cgggaaggac ttaattgcac aggcacggac aggaactggg aagacattct 781 cctttgccat ccctttgatt gagaaacttc atggggaact gcaagacagg aagagaggcc 841 gtgcccctca ggtactggtt cttgcaccta caagagagtt ggcaaatcaa gtaagcaaag 901 acttcagtga catcacaaaa aagctgtcag tggcttgttt ttatggtgga actccctatg 961 gaggtcaatt tgaacgcatg aggaatggga ttgatatcct ggttggaaca ccaggtcgta 1021 tcaaagacca catacagaat ggcaaactag atctcaccaa acttaagcat gttgtcctgg 1081 atgaagtgga ccagatgttg gatatgggat ttgctgatca agtggaagag attttaagtg 1141 tggcatacaa gaaagattct gaagacaatc cccaaacatt gcttttttct gcaacttgcc 1201 ctcattgggt atttaatgtt gccaagaaat acatgaaatc tacatatgaa caggtggacc 1261 tgattggtaa aaagactcag aaaacggcaa taactgtgga gcatctggct attaagtgcc 1321 actggactca gagggcagca gttattgggg atgtcatccg agtatatagt ggtcatcaag 1381 gacgcactat catcttttgt gaaaccaaga aagaagccca ggagctgtcc cagaattcag 1441 ctataaagca ggatgctcag tccttgcatg gagacattcc acagaagcaa agggaaatca 1501 ccctgaaagg ttttagaaat ggtagttttg gagttttggt ggcaaccaat gttgctgcac 1561 gtgggttaga catccctgag gttgatttgg ttatacaaag ctctccacca aaggatgtag 1621 agtcctacat tcatcgatcc gggcggacag gcagagctgg aaggacgggg gtgtgcatct 1681 gcttttatca gcacaaggaa gaatatcagt tagtacaagt ggagcaaaaa gcgggaatta 1741 agttcaaacg aataggtgtt ccttctgcaa cagaaataat aaaagcttcc agcaaagatg 1801 ccatcaggct tttggattcc gtgcctccca ctgccattag tcacttcaaa caatcagctg 1861 agaagctgat agaggagaag ggagctgtgg aagctctggc agcagcactg gcccatattt 1921 caggtgccac gtccgtagac cagcgctcct tgatcaactc aaatgtgggt tttgtgacca 1981 tgatcttgca gtgctcaatt gaaatgccaa atattagtta tgcttggaaa gaacttaaag 2041 agcagctggg cgaggagatt gattccaaag tgaagggaat ggtttttctc aaaggaaagc 2101 tgggtgtttg ctttgatgta cctaccgcat cagtaacaga aatacaggag aaatggcatg 2161 attcacgacg ctggcagctc tctgtggcca cagagcaacc agaactggaa ggaccacggg 2221 aaggatatgg aggcttcagg ggacagcggg aaggcagtcg aggcttcagg ggacagcggg 2281 acggaaacag aagattcaga ggacagcggg aaggcagtag aggcccgaga ggacagcgat 2341 caggaggtgg caacaaaagt aacagatccc aaaacaaagg ccagaagcgg agtttcagta 2401 aagcatttgg tcaataccca actttcttgt acaaagtggt tggtaagcct atccctaacc 2461 ctctcctcgg tctcgattct acgtagtaat gagctagcgc taaccggtgg cgcgttaagt 2521 cgacaatcaa cctctggatt a