Construct: ORF ccsbBroad304_02259
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005149.1_s304c1, BRDN0000411490
- Derived from:
- ccsbBroadEn_02259
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ELMO1 (9844)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_02259
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9844 | ELMO1 | engulfment and cell motility 1 | NM_001039459.2 | 100% | 100% | |
| 2 | human | 9844 | ELMO1 | engulfment and cell motility 1 | NM_130442.3 | 100% | 100% | |
| 3 | human | 9844 | ELMO1 | engulfment and cell motility 1 | NM_001206480.2 | 33.9% | 33.9% | 1_1440del |
| 4 | human | 9844 | ELMO1 | engulfment and cell motility 1 | NM_001206482.1 | 33.9% | 33.9% | 1_1440del |
| 5 | human | 9844 | ELMO1 | engulfment and cell motility 1 | NM_014800.10 | 33.9% | 33.9% | 1_1440del |
| 6 | human | 9844 | ELMO1 | engulfment and cell motility 1 | XM_005249919.3 | 33.9% | 33.9% | 1_1440del |
| 7 | human | 9844 | ELMO1 | engulfment and cell motility 1 | XM_006715805.1 | 33.9% | 33.9% | 1_1440del |
| 8 | human | 9844 | ELMO1 | engulfment and cell motility 1 | XM_011515654.2 | 33.9% | 33.9% | 1_1440del |
| 9 | human | 9844 | ELMO1 | engulfment and cell motility 1 | XM_017012839.1 | 33.9% | 33.9% | 1_1440del |
| 10 | human | 9844 | ELMO1 | engulfment and cell motility 1 | XM_024447008.1 | 33.9% | 33.9% | 1_1440del |
| 11 | human | 9844 | ELMO1 | engulfment and cell motility 1 | XR_001744894.2 | 16.8% | 1_1789del;1950_1951ins113;2418_3611del | |
| 12 | human | 9844 | ELMO1 | engulfment and cell motility 1 | NR_038120.1 | 16.8% | 1_347del;508_509ins113;976_3623del | |
| 13 | mouse | 140580 | Elmo1 | engulfment and cell motility 1 | NM_198093.3 | 92.8% | 100% | (many diffs) |
| 14 | mouse | 140580 | Elmo1 | engulfment and cell motility 1 | XM_017315380.1 | 36.3% | 39.1% | (many diffs) |
| 15 | mouse | 140580 | Elmo1 | engulfment and cell motility 1 | NM_080288.2 | 31.5% | 33.9% | (many diffs) |
| 16 | mouse | 140580 | Elmo1 | engulfment and cell motility 1 | XM_006516547.3 | 31.5% | 33.9% | (many diffs) |
| 17 | mouse | 140580 | Elmo1 | engulfment and cell motility 1 | XM_006516544.3 | 30.9% | 33.2% | (many diffs) |
| 18 | mouse | 140580 | Elmo1 | engulfment and cell motility 1 | XM_006516545.3 | 30.9% | 33.2% | (many diffs) |
| 19 | mouse | 140580 | Elmo1 | engulfment and cell motility 1 | XM_006516546.3 | 30.9% | 33.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 807
- ORF length:
- 741
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgca ggtggtgaag gagcaggtta tgagagcact tacaaccaag cctagctccc 121 tggaccagtt caagagcaaa ctgcagaacc tgagctacac tgagatcctg aaaatccgcc 181 agtccgagag gatgaaccag gaagatttcc agtcccgccc gattttggaa ctaaaggaga 241 agattcagcc agaaatctta gagctgatca aacagcaacg cctgaaccgc cttgtggaag 301 ggacctgctt taggaaactc aatgcccggc ggaggcaaga caagttttgg tattgtcggc 361 tttcgccaaa tcacaaagtc ctgcattacg gagacttaga agagagtcct cagggagaag 421 tgccccacga ttccttgcag gacaaactgc cggtggcaga tatcaaagcc gtggtgacgg 481 gaaaggactg ccctcatatg aaagagaaag gtgcccttaa acaaaacaag gaggtgcttg 541 aactcgcttt ctccatcttg tatgactcaa actgccaact gaacttcatc gctcctgaca 601 agcatgagta ctgtatctgg acggatggac tgaatgcgct actcgggaag gacatgatga 661 gcgacctgac gcggaatgac ctggacaccc tgctcagcat ggaaatcaag ctccgcctcc 721 tggacctgga aaacatccag atccctgacg cacctccgcc gattcccaag gagcccagca 781 actatgactt cgtctatgac tgtaactgcc caactttctt gtacaaagtg gttggtaagc 841 ctatccctaa ccctctcctc ggtctcgatt ctacgtagta atgagctagc gctaaccggt 901 ggcgcgttaa gtcgacaatc aacctctgga tta