Construct: ORF ccsbBroad304_03042
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010520.1_s304c1, BRDN0000407144
- Derived from:
- ccsbBroadEn_03042
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CCDC22 (28952)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_03042
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 28952 | CCDC22 | coiled-coil domain containi... | NM_014008.5 | 99.9% | 100% | 747A>G |
| 2 | human | 28952 | CCDC22 | coiled-coil domain containi... | XM_005272599.4 | 99.7% | 99.8% | 531_532insGGT;744A>G |
| 3 | human | 28952 | CCDC22 | coiled-coil domain containi... | XR_430506.3 | 83% | (many diffs) | |
| 4 | mouse | 54638 | Ccdc22 | coiled-coil domain containi... | NM_138603.3 | 85.7% | 88% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1947
- ORF length:
- 1881
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgga ggaggcggac cgaatcctca tccattcgct gcgccaggcc ggcacggcag 121 ttcctccaga tgtgcagacc ttgcgcgcct tcaccactga gctggttgta gaggctgtgg 181 tccgctgcct gcgtgtgatc aaccctgcgg tgggctctgg cctcagccct ctgctgcctc 241 ttgccatgtc tgcccggttc cgcctggcca tgagcctggc tcaggcctgc atggacctgg 301 gctatccctt ggagcttggc tatcagaact tcctctaccc cagtgagcct gacctccgag 361 acctgcttct cttcttggct gagcgtctgc ccaccgatgc ctctgaggat gcagaccagc 421 ctgcaggtga ctcagctatt ctcctccggg ccattgggag ccaaattcgg gaccagctgg 481 cactgccttg ggtcccgccc caccttcgca ctcccaagct gcagcacctc cagggctcgg 541 ccctccagaa gcctttccat gccagcaggc tggtcgtgcc agaattgagt tccagaggtg 601 agccacggga gttccaggcg agtcccctgc tgcttccagt ccctacccag gtgcctcagc 661 ctgttggaag ggtggcctcg ctcctcgaac accatgccct gcagctctgc cagcagacgg 721 gccgggaccg gccaggggat gaggactggg tccaccggac atcccgcctc ccaccccagg 781 aggacacacg ggctcagcgg cagcggctgc agaagcaact gactgagcat ctgcgccaaa 841 gctggggcct gcttggggcc cccatacaag cccgggacct gggagaactg ctgcaggcct 901 ggggtgctgg ggccaagact ggtgctccta agggctcccg cttcacgcac tcagagaagt 961 tcaccttcca tctggagccc caggcccagg ccactcaggt gtcagatgtg ccagccacct 1021 cccggcggcc tgaacaggtc acgtgggcag ctcaggaaca ggagctcgag tcccttcggg 1081 agcagctgga aggagtgaac cgcagcattg aggaggttga ggccgacatg aagaccctgg 1141 gcgtcagctt tgtgcaggca gagtctgagt gccggcacag caagctcagt acagcagagc 1201 gtgagcaggc cctgcgcctg aagagccgcg cggtggagct gctgcccgat gggactgcca 1261 accttgccaa gctgcagctt gtggtggaga atagtgccca gcgggtcatc cacttggcgg 1321 gtcagtggga gaagcaccgg gtcccactcc tcgctgagta ccgccacctc cgaaagctgc 1381 aggattgcag agagctggaa tcttctcgac ggctggcaga gatccaagaa ctgcaccaga 1441 gtgtccgggc ggctgctgaa gaggcccgca ggaaggagga ggtctataag cagctgatgt 1501 cagagctgga gactctgccc agagatgtgt cccggctggc ctacacccag cgcatcctgg 1561 agatcgtggg caacatccgg aagcagaagg aagagatcac caagatcttg tctgatacga 1621 aggagcttca gaaggaaatc aactccctat ctgggaagct ggaccggacg tttgcggtga 1681 ctgatgagct tgtgttcaag gatgccaaga aggacgatgc tgttcggaag gcctataagt 1741 atctagctgc tctgcacgag aactgcagcc agctcatcca gaccatcgag gacacaggca 1801 ccatcatgcg ggaggttcga gacctcgagg agcagatcga gacagagctg ggcaagaaga 1861 ccctcagcaa cctggagaag atccgggagg actaccgagc cctccgccag gagaacgctg 1921 gcctcctagg ccgggtccgg gaggcctgcc caactttctt gtacaaagtg gttggtaagc 1981 ctatccctaa ccctctcctc ggtctcgatt ctacgtagta atgagctagc gctaaccggt 2041 ggcgcgttaa gtcgacaatc aacctctgga tta