Construct: ORF ccsbBroad304_03143

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF004916.1_s304c1, BRDN0000410822
Derived from:
ccsbBroadEn_03143
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
SMARCAL1 (50485)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_03143
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 50485 SMARCAL1 SWI/SNF related, matrix ass... NM_001127207.2 100% 100%
2 human 50485 SMARCAL1 SWI/SNF related, matrix ass... NM_014140.4 100% 100%
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2928
ORF length:
2862
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgtc cttgcctctt acagaggagc agaggaaaaa gattgaagag aatcgacaaa
121aggctctggc ccgcagagct gagaagttat tggcagaaca gcatcagagg actagctcgg
181gcacctccat tgctggcaac ccattccagg ccaagcaagg cccatcccaa aatttcccaa
241gggagtcttg taagccagtg agccatggtg tcattttcaa gcaacagaat ctcagtagct
301catctaatgc tgaccaaaga cctcatgatt cccacagttt tcaggcaaag ggaatatgga
361aaaagccaga agaaatgccc acagcctgcc caggccacag tccacgtagt caaatggctc
421tcactggaat ctctcctccc ttggcacaaa gtcctccaga ggtccctaaa caacagctct
481tgagttatga gttaggtcaa ggtcatgctc aggcttcacc tgagatcagg ttcacaccct
541ttgctaaccc aactcataag cctctggcca aaccaaagag ttcccaagag acaccagctc
601attcctctgg acagcctccc agggatgcta agttagaggc caagacagca aaagcctccc
661cttcggggca gaacatttct tacatccatt ctagctcaga gagtgtaacg cccaggacag
721aaggaagact ccagcagaag tcagggtcct cagtccaaaa aggagtgaac tctcagaagg
781gaaagtgcgt aaggaacggc gatcgtttcc aggtgttgat tgggtacaat gcggaactca
841ttgcagtgtt taagaccctg cccagcaaga attatgatcc tgacaccaag acgtggaact
901tcagcatgaa tgactatagt gccctgatga aagcagccca gagcctcccc acggtcaacc
961tgcagcctct ggaatgggcc tatggcagca gcgagtcacc ctccaccagc agtgagggac
1021aggccggcct tccatcagct ccatcccttt catttgtcaa agggcgatgc atgctcatct
1081ccagggccta cttcgaggca gacatcagtt attcacagga ccttattgcg ctttttaaac
1141agatggattc cagaagatat gatgtcaaga ccaggaagtg gagctttctc ttggaagagc
1201acagtaaact aattgcaaag gtgcgctgcc tcccacaagt tcagctggac cctctgccca
1261cgactctcac cctggcgttt gcttctcagc tcaagaagac atctctcagt ctcacgccag
1321atgtcccaga ggcagacctt tctgaagtgg accccaagct cgtgtctaat ctgatgccct
1381ttcagagagc tggagtcaat tttgccatag ccaaaggagg ccgcctgctg ctcgctgacg
1441acatgggcct ggggaagacc atccaagcca tctgcatcgc agccttttac cggaaggagt
1501ggccgctcct ggtggtggtg ccatcctccg tgcgcttcac ctgggagcag gccttccttc
1561ggtggctgcc atctctgagc ccagattgca tcaacgtcgt ggtgactggg aaggaccgcc
1621tgacagctgg cctgatcaac attgtcagct ttgaccttct tagcaagttg gaaaaacagc
1681taaaaacccc ttttaaagtt gtcatcattg atgaatctca cttcctcaaa aacagtagga
1741ctgcccgctg tcgagcagct atgccggtcc taaaggttgc caagagggtg atcctgttgt
1801cgggcacacc agccatgtcc cggcccgcag agctctacac gcagatcatc gcagtcaagc
1861caactttctt cccccagttt catgcctttg gacttcgcta ctgtgatgcc aaacggatgc
1921cttgggggtg ggactactca ggttcctcca acctgggaga gctgaagctc ctgctggagg
1981aagcagtcat gctgcggcgc ctcaagtccg acgtcctttc ccagctgcct gccaagcagc
2041gcaagatagt ggtgattgcc ccaggacgga tcaatgccag gaccagagct gccctggatg
2101ctgcagccaa ggaaatgacc accaaggaca aaactaaaca gcagcagaaa gatgccctca
2161ttctcttctt caacagaaca gctgaagcta aaatcccatc tgtcattgaa tatatcttgg
2221acctactgga aagtggaaga gagaagtttt tagtatttgc acaccataag gtggtcctgg
2281acgcaattac gcaagagctt gagagaaagc acgtgcagca catccgcatc gatggctcca
2341cctcatcagc tgagcgggag gacctgtgcc agcagttcca actgtcggag aggcatgctg
2401tggccgtgct gtccatcacc gctgccaata tgggcctcac cttctcctcg gctgacctgg
2461tggtgtttgc tgagctgttt tggaacccag gggtgctgat ccaggctgag gaccgcgtgc
2521accgcattgg acagaccagc tccgtgggca ttcactacct cgtggcaaag ggcacagctg
2581atgactacct ttggcccctg attcaagaga agattaaagt tctggcagaa gccgggcttt
2641ctgagaccaa tttttcagaa atgacagaat ccactgatta cctctacaag gacccaaagc
2701agcagaagat ctacgaccta ttccagaagt cctttgagaa agaaggaagt gatatggagc
2761tcctggaagc agcagagtcc tttgacccag gaagtgcttc aggaacatct ggaagtagtt
2821cccagaacat gggagacacc ctggatgaaa gctcattgac agccagtcca cagaagaaaa
2881ggagatttga attttttgat aactgggaca gctttacgtc tcccctgtac ccaactttct
2941tgtacaaagt ggttggtaag cctatcccta accctctcct cggtctcgat tctacgtagt
3001aatgagctag cgctaaccgg tggcgcgtta agtcgacaat caacctctgg atta

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