Construct: ORF ccsbBroad304_03175
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008038.1_s304c1, BRDN0000410975
- Derived from:
- ccsbBroadEn_03175
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MRPS16 (51021)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_03175
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51021 | MRPS16 | mitochondrial ribosomal pro... | NM_016065.4 | 100% | 100% | |
| 2 | mouse | 66242 | Mrps16 | mitochondrial ribosomal pro... | NM_025440.2 | 83.9% | 89.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 477
- ORF length:
- 411
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggt ccacctcact actctcctct gcaaggccta ccgtgggggc cacttaacca 121 tccgccttgc cctgggtggc tgcaccaatc ggccgttcta ccgcattgtg gctgctcaca 181 acaagtgtcc cagggatggc cgtttcgtag agcagctggg ctcctatgat ccattgccca 241 acagtcatgg agaaaaactc gttgccctca acctagacag gatccgtcat tggattggct 301 gcggggccca cctctctaag cctatggaaa agcttctggg tcttgctggc tttttccctc 361 tgcatcctat gatgatcaca aatgctgaga gactgcgaag gaaacgggca cgtgaagtcc 421 tgttagcttc tcagaaaaca gatgcagaag ctacagatac agaggctaca gaaacatacc 481 caactttctt gtacaaagtg gttggtaagc ctatccctaa ccctctcctc ggtctcgatt 541 ctacgtagta atgagctagc gctaaccggt ggcgcgttaa gtcgacaatc aacctctgga 601 tta